The Murphy group has numerous transcriptional datasets from the kidney and brain of a variety of species under water restriction (and rehydration) conditions. The aim of this site is to make this data accessible. Choose your species in the drop down menu then use the search box to find how the expression of a gene or genes of interest change in dehydration and rehydration. To see all species' expression at once, search using the box on this tab, then move to the "multi Species/Tissue plots" tab.

Use gene symbols to search. Please note that in converting to human readable gene symbols, some genes may be lost as multiple Ensembl IDs may link to a single gene symbol. The annotations of non-model species (ie Camel and Jerboa) may not be as complete as model organisms. Therefore, some genenames will return NULL results.

To search by GeneSymbol ( regexp allowed: for example add ^ to define start and $ to define end ) simply add your term to the Gene Search box below and press Update Plot . An additional option allows you to only display the genes that were significant (padj<0.05) in either condition. Happy gene hunting! Note that if your search returns no results, you may have to reconnect to the server. The plots are faceted by GeneSymbol so when there are multiple Ensembl IDs linked to a single gene symbol (eg ATP11A), the expression data is combined into the boxplot (possibly creating boxes with large spreads) although the significance bars are kept separate. Work is ongoing for a search function using unique Ensembl IDs.



Technical notes: DESeq2 was used to calculate significance values.

Note the differences in experimental design when comparing datasets. Jerboa were water restricted for 10 days. Camel were water restricted for 21 days. Rats (Wistar) were water restricted for 2 days.

Jerboa SON data is split into three analyses. This is because there may be sex differences. The analyses are Additive which is the result for the model ~sex + condition (differences between conditions controlling for sex), Male is the analysis (~condition) with male samples only, and Female which unsurprisingly is the analysis with female samples only.

The error messages "data should contain group1 and group2 columns" and "'x' and 'units' must have length > 0" on the multispecies tab occur when there is no matching record for the search term entered. Try loosening the term using REGEX .

If there is no significance bar, this means that DESeq2 returned NA as the adjusted significance value.

This site was built and is maintained by Ben Gillard. Please report any bugs or suggest any improvements via bg14337@bristol.ac.uk